Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180529_wri6 TTCATAGTTCAGATGTCATAATGTTCACTAGG(+CAGTGT)GATCCTCAACCAGTATATAAAATATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605180528_s9zk GGGCTTGACTCTGATTGATGGCCTCTGGAGAG(+CAGTGT)GATCCTGCAGCTACCCAGATTCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605180527_ht64 TTGGAGCCAACCTTGAATCTGGGTAGCTGCAG(+CAGTGT)GATCCTCTCCAGAGGCCATCAATCAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605180525_wcml ACAGGGTAATTCATTAGCAAGACATTTCATTG(+CAGTGT)GTACCGGGTTCAGGTTGTCTGGGGAATAATG NGG 10 8 17 8 24 0 100 5 10
    240605180523_m542 CCTCCCATTATTCCCCAGACAACCTGAACCCG(+CAGTGT)GTACCAATGAAATGTCTTGCTAATGAATTAC NGG 10 8 17 8 24 0 100 5 10
    240605180520_v5xj CCCCCGGGTCCAGCTCTGCTCCTGTGCCTGGG(+CAGTGT)GATCCGGAGGCTCCTGGCTGTCTTGAAATGG NGG 10 8 17 8 24 0 100 5 10
    240605180518_77lf AAAGTCCATTTCAAGACAGCCAGGAGCCTCCG(+CAGTGT)GATCCCCAGGCACAGGAGCAGAGCTGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605180515_3x7g AGGTGTGGCTGTAAGCTTCCAGGCTTCCCCTG(+CAGTGT)GTACCCGGCATGCCAGGGTAGCCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240605180513_kf9u TCCTGCAGGTGCAGGCTACCCTGGCATGCCGG(+CAGTGT)GTACCAGGGGAAGCCTGGAAGCTTACAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605180512_k45s GCACCCTGTGAGTGTAGAATGTTTTGTAAGTG(+CAGTGT)GATCCTCTTTCCTTGAAATCTGTAAGCTCAA NGG 10 8 17 8 24 0 100 5 10