Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180424_cojh CTAAGCAGCGGGGCTCTAGGGCACCTGAACCG(+CAGTGT)GATCCGTGATGAACTAATAGGCCTTGCCCGC NGG 10 8 17 8 24 0 100 5 10
    240605180421_xhbg CCCTGACAAAACTCATTCTACCCAGTACCCAG(+CAGTGT)GATCCTGGGCACACGCACATCTATATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240605180419_qz64 AACCATTTCCATATAGATGTGCGTGTGCCCAG(+CAGTGT)GATCCTGGGTACTGGGTAGAATGAGTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240605180417_ytj4 GAGGATTGACTCCTCAGAGAAGGAGCCTATGG(+CAGTGT)GTACCTGGGGCCACGTACAGCAGGGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605180413_51sc CTGCCGCTGAGCCCTGCTGTACGTGGCCCCAG(+CAGTGT)GTACCCATAGGCTCCTTCTCTGAGGAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605180412_qcyd CTTGGAAAGATGGGCCACATGCTGCATGACAG(+CAGTGT)GATCCTCCAAGTACCTTAACTGTTAGAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605180410_x4e1 CACGGATACTCTAACAGTTAAGGTACTTGGAG(+CAGTGT)GATCCTGTCATGCAGCATGTGGCCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240605180409_8sy6 AGTGACCCTCCCAACACCTCTGTGCCAGCAGG(+CAGTGT)GATCCCCAAGGCAGCTACCACCTTAACATCT NGG 10 8 17 8 24 0 100 5 10
    240605180407_i7nk GTCCTAGATGTTAAGGTGGTAGCTGCCTTGGG(+CAGTGT)GATCCCTGCTGGCACAGAGGTGTTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240605180406_5pi8 GACCTCTTCCTGTGAATGTGACCCATTCATTG(+CAGTGT)GTACCTGAAGTTATTAGTAGTATCCAATGCC NGG 10 8 17 8 24 0 100 5 10