Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180404_n0lc AACAGGGCATTGGATACTACTAATAACTTCAG(+CAGTGT)GTACCAATGAATGGGTCACATTCACAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605180403_ilnt GAGAATTTTCTCTCCTCCTCCACTGGGGACAG(+CAGTGT)GATCCACCAGATGTCAATAGCGTGCTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605180401_woux AGCGAAAATAAGCACGCTATTGACATCTGGTG(+CAGTGT)GATCCTGTCCCCAGTGGAGGAGGAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605180359_y5y1 CGGCACGGCCCGCCCGGCCCCTCCACCAGGAG(+CAGTGT)GATCCGAAAGGTTACCTGTACAGTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605180358_y6ns AGGCTACAGGAAACTGTACAGGTAACCTTTCG(+CAGTGT)GATCCTCCTGGTGGAGGGGCCGGGCGGGCCG NGG 10 8 17 8 24 0 100 5 10
    240605180356_pz9l TGGAGTCCACTGCTGGCCAGCCCCAGCACTGG(+CAGTGT)GTACCCACAGGACATGCCCCCACACACCTCA NGG 10 8 17 8 24 0 100 5 10
    240605180355_29ev TGTTGTGAGGTGTGTGGGGGCATGTCCTGTGG(+CAGTGT)GTACCCAGTGCTGGGGCTGGCCAGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605180353_6kg8 TGTAGTCCCTCTTATCCGGCTGGATTAACCTG(+CAGTGT)GTACCCCAACATTATCCAGATTTCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240605180350_9xxp GTTTTGGAGTAGGAAATCTGGATAATGTTGGG(+CAGTGT)GTACCAGGTTAATCCAGCCGGATAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605180349_jltx CATCTCTGGAACTCGGATTCCCATCGTATTGG(+CAGTGT)GTACCACTGCTTTTAAAGCACTAAGTGTTAA NGG 10 8 17 8 24 0 100 5 10