Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180454_mpd5 TGTTCTTGGTTGGCCCCTGGGCTTAGCTCTTG(+CAGTGT)GTACCTTTGGTGTTTTCTCTGAGGTGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240605180453_m3j5 TAACTCCCAGAATCAAAAGGGCCCGAGGGAGG(+CAGTGT)GTACCTGTGAACGGTTCCCGCGGGTGGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605180451_cb5f TTCTTAGACCCACCCGCGGGAACCGTTCACAG(+CAGTGT)GTACCCTCCCTCGGGCCCTTTTGATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605180450_ictw CAGGCTGGCTGCGAAAGGAGGCTACACATATG(+CAGTGT)GATCCACCTGACTGTAATCAGTGAATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240605180449_n3wl AGAAGTTGAGATTCACTGATTACAGTCAGGTG(+CAGTGT)GATCCATATGTGTAGCCTCCTTTCGCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605180448_8f9u GCTGGGGAGGCCAGTGTATCTGCTGGAGTCTG(+CAGTGT)GATCCTGACATTTACCTATTTCTTATTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605180447_07ey AGGATTGCCAATAAGAAATAGGTAAATGTCAG(+CAGTGT)GATCCAGACTCCAGCAGATACACTGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605180445_oy7w AGTCCCAACACCTATGCGTGTTCCCAACTCCG(+CAGTGT)GATCCTGTTAATACTATGGTTAAAAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240605180443_wmj7 TATGGAACCACTTTTAACCATAGTATTAACAG(+CAGTGT)GATCCGGAGTTGGGAACACGCATAGGTGTTG NGG 10 8 17 8 24 0 100 5 10
    240605180442_uchw TCTGGGCAGTAAGGACCCTATCACTGAGCCAG(+CAGTGT)GTACCTTGTCCTCTTCTTATTTTGAAGCATC NGG 10 8 17 8 24 0 100 5 10