Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180347_fdoh TATGTTTAACACTTAGTGCTTTAAAAGCAGTG(+CAGTGT)GTACCCAATACGATGGGAATCCGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240605180346_81j0 GAGCATCCTCTGTCCTGCCTCGTGCCCAGATG(+CAGTGT)GTACCTCTGGAGAAGGCATGCGGCTGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605180344_bhon CAGATGAGGCCAGCCGCATGCCTTCTCCAGAG(+CAGTGT)GTACCATCTGGGCACGAGGCAGGACAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605180342_bubg GGCCGCTCTGTTCCCCCTGACCTAGCCTCGGG(+CAGTGT)GTACCCTGTGGCCATTTGTGGTGACTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605180341_1nos GGAGAGGCAAAGTCACCACAAATGGCCACAGG(+CAGTGT)GTACCCCGAGGCTAGGTCAGGGGGAACAGAG NGG 10 8 17 8 24 0 100 5 10
    240605180339_lxx0 ACACCAGGCACACAGAGAGAAGTTCATGCCAG(+CAGTGT)GTACCAAGGGAAGATGTGAGACAGAGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605180336_eajv AGAGGGCTGGCTCTGTCTCACATCTTCCCTTG(+CAGTGT)GTACCTGGCATGAACTTCTCTCTGTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605180335_ol1b CAGCCTAAGAATCAAGCTAAGGTATTATCAGG(+CAGTGT)GATCCCAAATGCCCCTCCCACCCAGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605180333_b258 TAACGTCCTTCCTGGGTGGGAGGGGCATTTGG(+CAGTGT)GATCCCTGATAATACCTTAGCTTGATTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605180331_f3mu GGGCTCAGGCTCTTGTTGTGGGCGCTGCCTGG(+CAGTGT)GATCCATTCCTGAGGAGCTGGGCTAGATAGA NGG 10 8 17 8 24 0 100 5 10