Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180255_t0f9 AGGGTGGCACAGCAGCCTGGCGTCAGTGACTG(+CAGTGT)GTACCCCTTCTCCTGTGTCCAGTGCAGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605180253_xxzs ACAAGAAAGCCGCTCTCGTCTGCAGGACACGG(+CAGTGT)GATCCTGCGTGGCTCACAGCGGCAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605180251_qrg2 AGAAGTGGCAGCTGCCGCTGTGAGCCACGCAG(+CAGTGT)GATCCCGTGTCCTGCAGACGAGAGCGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605180250_w4dr CCAGTTTTTCTCAGATTTAGGGAGAATACATG(+CAGTGT)GTACCTTTTACCTACCCATACTAGAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240605180249_hndv TGTAGTTAGTTTCTAGTATGGGTAGGTAAAAG(+CAGTGT)GTACCATGTATTCTCCCTAAATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605180247_o6ep AAGTGGGGAGGAATGGTACTATTGGCATGGGG(+CAGTGT)GTACCTGGTATTGAAAATGGACACATAAACT NGG 10 8 17 8 24 0 100 5 10
    240605180245_g7l9 CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+CAGTGT)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605180244_kbd6 GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+CAGTGT)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240605180243_hq9i TATTTGTGTATGTGTACACCATGTGTGTGCAG(+CAGTGT)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605180242_md5j GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+CAGTGT)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10