Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180207_vlng AGAGACTTCAGGATGGGGGTGGGGCCCTCCAG(+CAGTGT)GATCCCACGGGCACCAAATCCAGACTCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605180206_eu54 CAATCCCAGCATTCAAGAGGTAGAGGCAGGAG(+CAGTGT)GATCCGGAGTTCAAAGTCATCCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605180204_11jr CTGTTTCCAGCCAAGGATGACTTTGAACTCCG(+CAGTGT)GATCCTCCTGCCTCTACCTCTTGAATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605180203_hg9x CAGCCCTCCCCAGAACAGTTGACTGGGACAAG(+CAGTGT)GATCCTGAGCATGTCTAGGATCTGCAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605180201_zk73 GTTAGGTGTTTGCAGATCCTAGACATGCTCAG(+CAGTGT)GATCCTTGTCCCAGTCAACTGTTCTGGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605180159_ag0m AGGATGCCTTCCTATGATAGCTCGGGTTCCTG(+CAGTGT)GTACCCCTTTCTCCCCGTGCATGGGAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240605180157_b5vk ACTGCATCTCTCCCATGCACGGGGAGAAAGGG(+CAGTGT)GTACCAGGAACCCGAGCTATCATAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605180156_gc7v CTGGGTACAGAAAGAACAGAACAGGTTGCTAG(+CAGTGT)GTACCTGTCCACTAAAATCTATAGCTATTTG NGG 10 8 17 8 24 0 100 5 10
    240605180155_palp CAGAACAAATAGCTATAGATTTTAGTGGACAG(+CAGTGT)GTACCTAGCAACCTGTTCTGTTCTTTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605180153_apal GACCACGACCGCCTTCAACTCCAGCCTCAAGG(+CAGTGT)GATCCAATGGCATCTGCTGCATGCGTTCATG NGG 10 8 17 8 24 0 100 5 10