Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180104_sng8 TAGAAATGGAACACAATGATGCAATGTATCTG(+CAGTGT)GATCCCGTATGATTTTGTTTCGCTTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240605180102_dlyq ACCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605180101_lbg3 GCTGTCTTCTGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605180059_w4uz CCCATAGTAATGACCCTTAAAGTTGACCTCAG(+CAGTGT)GATCCACATACCAAGAAAAGAAATTGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605180058_j13p CTCTAGCTCTCAATTTCTTTTCTTGGTATGTG(+CAGTGT)GATCCTGAGGTCAACTTTAAGGGTCATTACT NGG 10 8 17 8 24 0 100 5 10
    240605180057_yufg ATTTTAAAATTAAGGCAGAGAGCACGAAGGAG(+CAGTGT)GTACCCAGGTCAGCCGACGGCCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240605180054_n3cb TGCTTGTGTGTGGAGGCCGTCGGCTGACCTGG(+CAGTGT)GTACCTCCTTCGTGCTCTCTGCCTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240605180052_9dtv TGCTCTGCCTCTCTGCTCAGGAACTAAGGATG(+CAGTGT)GTACCCTTTATCCAAAGCAGGAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605180051_pho2 GTTTCCTCACAACTTCCTGCTTTGGATAAAGG(+CAGTGT)GTACCATCCTTAGTTCCTGAGCAGAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605180049_lzxi CAAAGGTCTAGAAAGGAAGGTTGCTTGGGCTG(+CAGTGT)GATCCCTTCCCCTCCTTTTCTCCGATTAAAT NGG 10 8 17 8 24 0 100 5 10