Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180032_j3k7 ACTCAAGCAGGGCAAGGACTCAAGCAGGGCAG(+CAGTGT)GATCCTGGAGGCAGGAGCTGATGCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605180030_qbec GACACAGGGTGCTAAGTCTCACTGAACACTGG(+CAGTGT)GTACCCTAATAAGACTCAAACAGGCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240605180029_jpek GAGGGGATGGAGCCTGTTTGAGTCTTATTAGG(+CAGTGT)GTACCCAGTGTTCAGTGAGACTTAGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240605180026_ndrw GCTCTAGGCTGGTGGGAGAGGGGGTGTCCTGG(+CAGTGT)GATCCCCTCCAGGGACAAGTGCTCTGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605180024_colh AACTTCCCCCCAGAGCACTTGTCCCTGGAGGG(+CAGTGT)GATCCCAGGACACCCCCTCTCCCACCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605180023_qtv6 CGTTGGATACATTGCCACAGTAACTGAAGAAG(+CAGTGT)GTACCCGGAGCCCAGTGTGAATCGTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240605180021_qjse TTGGAATATAAACGATTCACACTGGGCTCCGG(+CAGTGT)GTACCTTCTTCAGTTACTGTGGCAATGTATC NGG 10 8 17 8 24 0 100 5 10
    240605180019_k0rw CCAGAGCCAAACCCTGTAGGTGCCCAGTGTGG(+CAGTGT)GATCCAGTATCTCATTTGGTGGTATCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240605180018_gfpg CACCAATAAAGATACCACCAAATGAGATACTG(+CAGTGT)GATCCCACACTGGGCACCTACAGGGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605180016_px7d TTGCACTGCAAACTTGAGTTCTTTACTGAAGG(+CAGTGT)GTACCAATGCATCCATCCATAAAGGTCCAGA NGG 10 8 17 8 24 0 100 5 10