Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180048_8n77 TTCGTATTTAATCGGAGAAAAGGAGGGGAAGG(+CAGTGT)GATCCAGCCCAAGCAACCTTCCTTTCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240605180046_gzmz CGCTAACACATACCCTCAGCCCCTCACTGGGG(+CAGTGT)GATCCCAAAGTGCTCTACATCTCTAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240605180044_kby3 TTTGAAAGATCTAGAGATGTAGAGCACTTTGG(+CAGTGT)GATCCCCCAGTGAGGGGCTGAGGGTATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605180042_mury CATTTCTCTCCATTTACCACGTGGCTCCTGAG(+CAGTGT)GATCCAACTCAGGTTATCAGGTAGGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605180041_nmaf ACACTTAACCACCTACCTGATAACCTGAGTTG(+CAGTGT)GATCCTCAGGAGCCACGTGGTAAATGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605180039_rlv7 CACCAGGAGCCATTCCTGGCTGCATCCCATTG(+CAGTGT)GTACCCAAATACCGGAAGCCTTGACGATGGA NGG 10 8 17 8 24 0 100 5 10
    240605180037_37cz CCAAATCCATCGTCAAGGCTTCCGGTATTTGG(+CAGTGT)GTACCAATGGGATGCAGCCAGGAATGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605180036_2w9x TTCTTGATCAAAGCCACTTTAGTGAGAAATAG(+CAGTGT)GTACCAGTCCTCAGGACGAGCCAGGATCCCT NGG 10 8 17 8 24 0 100 5 10
    240605180035_q6g7 CCCTGAGGGATCCTGGCTCGTCCTGAGGACTG(+CAGTGT)GTACCTATTTCTCACTAAAGTGGCTTTGATC NGG 10 8 17 8 24 0 100 5 10
    240605180033_9pqg TCCATGGCCTCTGCATCAGCTCCTGCCTCCAG(+CAGTGT)GATCCTGCCCTGCTTGAGTCCTTGCCCTGCT NGG 10 8 17 8 24 0 100 5 10