Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180137_vshd TACCCGCCAGCTGGTCAGGAGTCTGCACGTTG(+CAGTGT)GTACCCCTTTTCCCTGAACTGGGAGTAATCG NGG 10 8 17 8 24 0 100 5 10
    240605180135_mb8x CTGCCTCCAGAACACTGCAGGATTCTCCACCG(+CAGTGT)GTACCTGCTGGTTTCCCATAGGCCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605180134_kf08 CTGAGCAGCCTGGGCCTATGGGAAACCAGCAG(+CAGTGT)GTACCGGTGGAGAATCCTGCAGTGTTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605180132_kjzv GAAGACAGAGCCCACACAGTAGGAAATTCCAG(+CAGTGT)GTACCAACACTGTAAAGTATGTGTACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605180131_p9ww TCTCCTCTCAGTACACATACTTTACAGTGTTG(+CAGTGT)GTACCTGGAATTTCCTACTGTGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240605180129_jqmf TCTGTGCAGCAAAATGCTGGGTACTGTTTATG(+CAGTGT)GATCCAGTCTGTTAGTCTATGTCTCTTTATT NGG 10 8 17 8 24 0 100 5 10
    240605180128_mdth TCCACAATAAAGAGACATAGACTAACAGACTG(+CAGTGT)GATCCATAAACAGTACCCAGCATTTTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605180126_gkjv AGACGAATGCCGAAGCCTGGCATCAGGGACGG(+CAGTGT)GATCCCATCCTCTGGAGTAGGCAATAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240605180125_4gjf GTTTTCCACCTATTGCCTACTCCAGAGGATGG(+CAGTGT)GATCCCGTCCCTGATGCCAGGCTTCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240605180123_hy1a AGGGGTTCCAGATGCTCAGCAGACACTCCTGG(+CAGTGT)GATCCAAGGAAGCACTTATGCCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10