Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175957_7udb CCACATGTAGATCCACATACCACATACATGTG(+CAGTGT)GTACCCGCAGGGGCCAAAAGGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605175955_piy9 TCTCAATTAGTACTTATAGGGATGGGATTGCG(+CAGTGT)GTACCACGGATACTGAAGCCCTCCCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240605175953_bgxg GTCTAGAGTCAGGGAGGGCTTCAGTATCCGTG(+CAGTGT)GTACCGCAATCCCATCCCTATAAGTACTAAT NGG 10 8 17 8 24 0 100 5 10
    240605175951_1o3x AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+CAGTGT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605175950_2uhu GTGATATTTGTAAATAAAGGTAGAAACATTGG(+CAGTGT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605175948_x9ai AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+CAGTGT)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240605175946_j1go GTGATATTTGTAAATAAAGGTAGAAACATTGG(+CAGTGT)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605175945_4y9e TTTTTTCTTCCATCCCAAGTAAAAGAGGGTTG(+CAGTGT)GATCCTGTGTGTTCCTAGCAACAGCACCATT NGG 10 8 17 8 24 0 100 5 10
    240605175944_qbji TGAGCAATGGTGCTGTTGCTAGGAACACACAG(+CAGTGT)GATCCAACCCTCTTTTACTTGGGATGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605175942_v5sv TAACTCTCTCATGTCTGAGCAGTTCTCTTTCG(+CAGTGT)GATCCACTCTGCCTCGCTGCTGAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10