Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175941_rysl TTGAGTCTCTGACTCAGCAGCGAGGCAGAGTG(+CAGTGT)GATCCGAAAGAGAACTGCTCAGACATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605175940_zb6c AAAAAAAAAATAAATGTTTTCAAGTGTGTTGG(+CAGTGT)GTACCTAAGGGTCTTTGACAACCCCAGTACC NGG 10 8 17 8 24 0 100 5 10
    240605175938_iirt GCAAAGGTACTGGGGTTGTCAAAGACCCTTAG(+CAGTGT)GTACCCAACACACTTGAAAACATTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240605175937_k0d2 GTATGCTGTGACCTACTTTCAGGTGTTAAACG(+CAGTGT)GATCCTGCGTAGCTTCCCTGAGCACTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605175935_s81r AGAGGCAAAGAGTGCTCAGGGAAGCTACGCAG(+CAGTGT)GATCCGTTTAACACCTGAAAGTAGGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605175933_t18l CCCTCCAGGCTGCTGATACCCACTTAGAGAGG(+CAGTGT)GATCCCTCCAGACACCCCCCCACTCTGACAC NGG 10 8 17 8 24 0 100 5 10
    240605175931_16r1 ACAAAGTGTCAGAGTGGGGGGGTGTCTGGAGG(+CAGTGT)GATCCCTCTCTAAGTGGGTATCAGCAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605175930_q5gy GAGAAGAGAAAGAGATCTCGGGACGTCTGTGG(+CAGTGT)GTACCTTCATTCAGAAATACAATGGACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605175929_oleu CCCACCCTTGTCCATTGTATTTCTGAATGAAG(+CAGTGT)GTACCCACAGACGTCCCGAGATCTCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605175927_7y9f CTGCGCCCTGCACAGCAGACAATGTGTTCTGG(+CAGTGT)GTACCCCCAGCCTTTGCCATCTCAGGTCAAG NGG 10 8 17 8 24 0 100 5 10