Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175841_fg2p ACCTTTCATCTGGCCGAGCCAGGGGCAAAGGG(+CAGTGT)GATCCTAAGCAAATGCCTTCCGTGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240605175840_pmb7 TTACATGATAAGAAAGCTTGACACAAGCTGAG(+CAGTGT)GTACCAAGAGGCTCCTGCCACCTGGTCCACG NGG 10 8 17 8 24 0 100 5 10
    240605175839_9cd4 TCACCCGTGGACCAGGTGGCAGGAGCCTCTTG(+CAGTGT)GTACCTCAGCTTGTGTCAAGCTTTCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240605175838_gyby ACCCTAAACTCAAGTCAAGGTTAATGCATGCG(+CAGTGT)GATCCCGTGACGGGGCTCAGTGAGGCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605175835_69sr CCCTGGCAGGGCCTCACTGAGCCCCGTCACGG(+CAGTGT)GATCCGCATGCATTAACCTTGACTTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605175834_2lqs GACTCTGGGCACGCCCTTGGCTCCCTCGGAGG(+CAGTGT)GTACCGGCTCCTCCAGAGACTAGGTTTATTT NGG 10 8 17 8 24 0 100 5 10
    240605175832_hmqz GCAAAAAATAAACCTAGTCTCTGGAGGAGCCG(+CAGTGT)GTACCCTCCGAGGGAGCCAAGGGCGTGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605175831_4eev CTAAAGGGTGGTGACTGGCTTCAGGGGGTGTG(+CAGTGT)GTACCATCTGTGCCTGCTGCCAAGACGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605175830_ia14 TTGATCCTGCGTCTTGGCAGCAGGCACAGATG(+CAGTGT)GTACCACACCCCCTGAAGCCAGTCACCACCC NGG 10 8 17 8 24 0 100 5 10
    240605175828_2sxl GGAAGTATAGCCAAGATAGGCCCTGGGTCATG(+CAGTGT)GTACCTGCTCACTGGAGGTCTGCAAAGTAGG NGG 10 8 17 8 24 0 100 5 10