Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175856_squh AATGATGTCCACTTAGATTCTTAGGGGACATG(+CAGTGT)GTACCCATTTTCTGGTAGAGTTCAGTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605175854_vda1 CAGGATAAAAAAAAACCACATTCTTCCGGGAG(+CAGTGT)GTACCCTTTACCGGGCTCCCTGAGTGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605175852_9fqu TTCTCTGCAGCACTCAGGGAGCCCGGTAAAGG(+CAGTGT)GTACCTCCCGGAAGAATGTGGTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605175851_uv4d GGCACTTCAGAGAAAAACTAATGACAGCCTCG(+CAGTGT)GTACCTCGAACCTCATCGTTGTCTTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605175850_234n AGTTGAGAAAGAAGACAACGATGAGGTTCGAG(+CAGTGT)GTACCGAGGCTGTCATTAGTTTTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605175848_wpbe GGTTTGGAGAAAGATCCACACGTCAAAAGGTG(+CAGTGT)GTACCTGAATATGCACCAATTAGCAAACGAA NGG 10 8 17 8 24 0 100 5 10
    240605175847_44jn TTTGTTTCGTTTGCTAATTGGTGCATATTCAG(+CAGTGT)GTACCACCTTTTGACGTGTGGATCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605175846_vuk2 AAAAGCCCCTATGGAAAGCAAATTCCTGATTG(+CAGTGT)GTACCACCCTCGAATAGCTTATATCGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605175844_vuva CTCTATTTAACGATATAAGCTATTCGAGGGTG(+CAGTGT)GTACCAATCAGGAATTTGCTTTCCATAGGGG NGG 10 8 17 8 24 0 100 5 10
    240605175843_wemm GGGCCTGTTGTCCACGGAAGGCATTTGCTTAG(+CAGTGT)GATCCCCTTTGCCCCTGGCTCGGCCAGATGA NGG 10 8 17 8 24 0 100 5 10