Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175812_a8q1 CACTCCCTATAGTCATGATGGTCCGGATGCGG(+CAGTGT)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605175810_qjsk GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+CAGTGT)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605175809_zbuf TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+CAGTGT)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605175808_fa0c GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+CAGTGT)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605175806_3vag TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+CAGTGT)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240605175805_6q2u GTGTGTGGTTTCCATCCCAAGGATGCCTGGTG(+CAGTGT)GTACCACGGCTCCCAGGAAGAAGGTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605175803_nck1 CACACTCTCCAACCTTCTTCCTGGGAGCCGTG(+CAGTGT)GTACCACCAGGCATCCTTGGGATGGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240605175802_zep0 GCCACCCAGACTGTGGTAGCTTGGCATGAATG(+CAGTGT)GTACCGGCTGTTCTTATGAGTGTGAATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605175800_g835 CACATACACATTCACACTCATAAGAACAGCCG(+CAGTGT)GTACCATTCATGCCAAGCTACCACAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605175758_wlb1 CCTCGCCCCTGTGGGAAGGCAGGGCCAAAAAG(+CAGTGT)GTACCACATTCTCACTCAAAAAAAAACAAAC NGG 10 8 17 8 24 0 100 5 10