Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175725_6u2n AGCAAGCCATGAGATGGGTGTGTACTCTGAGG(+CAGTGT)GATCCATCCTCATCTCACATAGAAACTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605175724_865q ACTGTTACACGTATTGATGTTGGGTAGCTTGG(+CAGTGT)GATCCTTCTAGATCTCTGATGCAAGAAACCC NGG 10 8 17 8 24 0 100 5 10
    240605175723_5h0c AGTCTGGGTTTCTTGCATCAGAGATCTAGAAG(+CAGTGT)GATCCCAAGCTACCCAACATCAATACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605175721_jv2w GTACAGTGGATCTGAGCGGCAGGGGATTATTG(+CAGTGT)GTACCATCTGCTCTTCGGGCCCAACGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240605175720_76r6 GGGACTACCCCGTTGGGCCCGAAGAGCAGATG(+CAGTGT)GTACCAATAATCCCCTGCCGCTCAGATCCAC NGG 10 8 17 8 24 0 100 5 10
    240605175717_yj1g TGCCTGGTTCTAGGCCCCTTCCTCTACCCCAG(+CAGTGT)GTACCCTCAGCCTAGGGGAGCAGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605175716_15a0 CTTCCACCTGAGCCTGCTCCCCTAGGCTGAGG(+CAGTGT)GTACCTGGGGTAGAGGAAGGGGCCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605175715_np6f CCCCCGGGAACCCCCACCCCCACCCCCGGCAG(+CAGTGT)GATCCCTCCATGCAGGAGAAAGCCAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240605175713_n6nw GGGCCTCTGGTTGGCTTTCTCCTGCATGGAGG(+CAGTGT)GATCCTGCCGGGGGTGGGGGTGGGGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605175710_7t8j TGTCGCCCAGGGCTGTAAGCTGAGACCCCTGG(+CAGTGT)GATCCCTGTCTAAGCTAGATGCCCCTTCCCT NGG 10 8 17 8 24 0 100 5 10