Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175630_iped CCCCTCTCCTTCTATCTTCTCTCCCAACCCTG(+CAGTGT)GATCCTCAAGGTCTTTGCACTAGAAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240605175628_j6ih GAAAGAAGCTAGGACATGTCAAGAAATGGAGG(+CAGTGT)GATCCCAGGCTGCCCCCAACAAAATGGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605175626_9368 ACGTGGGGCCCATTTTGTTGGGGGCAGCCTGG(+CAGTGT)GATCCCTCCATTTCTTGACATGTCCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605175625_g71e CCTTTCTCCACAAGCAAGGCATGGCGTACCAG(+CAGTGT)GATCCCCACACTGTCCTGGCCTACTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240605175623_o6rt TGAAGGTAGAAAGTAGGCCAGGACAGTGTGGG(+CAGTGT)GATCCTGGTACGCCATGCCTTGCTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240605175622_br8a GGACTCGAGTGGCTCACAACCATCTGTAATAG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605175621_ycqg GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCTATTACAGATGGTTGTGAGCCACTCG NGG 10 8 17 8 24 0 100 5 10
    240605175618_pg3g ATACCAGGACCCACGTGTGCTAGTGTCCCTGG(+CAGTGT)GATCCCAGTTCACAGAAGAGCACCAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605175616_ksy5 CTTCCAGGCCTTGGTGCTCTTCTGTGAACTGG(+CAGTGT)GATCCCAGGGACACTAGCACACGTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605175613_56n3 CCTTAAGCTTATCTGCCTATTGATATCCCAGG(+CAGTGT)GATCCTGAAACAGGCCCCTGCGCATACAGAG NGG 10 8 17 8 24 0 100 5 10