Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175540_hifm TGGTGTTGTGGGAAGGTGTGAAGGACCCACTG(+CAGTGT)GTACCCAAGAGAGACATTCTCCTTGCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605175539_rjmm GCACACACTCCCAAAGAAGTTTCTGAGGAATG(+CAGTGT)GATCCTTGATTTTAAAACTGTAAGATCTTTT NGG 10 8 17 8 24 0 100 5 10
    240605175537_vwzl GGAGGAAAAGATCTTACAGTTTTAAAATCAAG(+CAGTGT)GATCCATTCCTCAGAAACTTCTTTGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605175536_cqtf TACAGGGATGCCACCACCATTCCAGCATATTG(+CAGTGT)GATCCTGATTCTTATATCCTGTTTGTGTGTT NGG 10 8 17 8 24 0 100 5 10
    240605175535_snrh ACTGAAACACACAAACAGGATATAAGAATCAG(+CAGTGT)GATCCAATATGCTGGAATGGTGGTGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240605175533_8se8 AGGCACAGTATTTGGTTAAGCCTGAGTTTTGG(+CAGTGT)GTACCTTTTGTTACTGTTTTCAAAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605175532_89ck GCAAGTCAGGGTTTTGAAAACAGTAACAAAAG(+CAGTGT)GTACCCAAAACTCAGGCTTAACCAAATACTG NGG 10 8 17 8 24 0 100 5 10
    240605175530_gare CCCAAGCCAAGCCTCAGCCTGGGCTTTCCCAG(+CAGTGT)GATCCTGTTAGTGAGCAGGGCTCAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240605175528_qs61 GGTCTGAGTGGCTGAGCCCTGCTCACTAACAG(+CAGTGT)GATCCTGGGAAAGCCCAGGCTGAGGCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605175527_d75u TGCAGCCACTGCCAGAGCTGACCTTGACTGGG(+CAGTGT)GATCCTTGTCTGGGACCCCATCACGCTGGTC NGG 10 8 17 8 24 0 100 5 10