Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175525_3g6q TAGAGGACCAGCGTGATGGGGTCCCAGACAAG(+CAGTGT)GATCCCCAGTCAAGGTCAGCTCTGGCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605175523_fcax TAACTTGTTTCATTTCTTTTCAGATCCCTAAG(+CAGTGT)GATCCCCCCACCCCCCAATGCCGCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240605175521_h1wi TTCAGAGTTGGAGCGGCATTGGGGGGTGGGGG(+CAGTGT)GATCCTTAGGGATCTGAAAAGAAATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605175519_v6j7 TTCAGAGCCAGCACATCACCATCTGTTTCTGG(+CAGTGT)GTACCTACCTTATGCCTACCAGAGGGCTCGT NGG 10 8 17 8 24 0 100 5 10
    240605175518_pslg ATAAAACGAGCCCTCTGGTAGGCATAAGGTAG(+CAGTGT)GTACCCAGAAACAGATGGTGATGTGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605175516_u5qj GGTGTCTGACCCTCCACCACCTCACCTTGTTG(+CAGTGT)GTACCTCCCTCCCTGAATTCCTGAGCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605175515_rtgb AGAGTTGCTGGCTCAGGAATTCAGGGAGGGAG(+CAGTGT)GTACCAACAAGGTGAGGTGGTGGAGGGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605175513_8ixj TGTCCCTCAGTATTGAACTTGGCTGCTTGTGG(+CAGTGT)GATCCAGTCCTGGAGGACAGGCATCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605175512_489r GCTAAGAACTTGATGCCTGTCCTCCAGGACTG(+CAGTGT)GATCCCACAAGCAGCCAAGTTCAATACTGAG NGG 10 8 17 8 24 0 100 5 10
    240605175511_st5d TTTGCTCTATTCCAGAGTCTGAGAGGTGTTTG(+CAGTGT)GTACCCGGTGTATCAGTAAATACTGCCTAGC NGG 10 8 17 8 24 0 100 5 10