Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175418_jk78 GGGTTCTTGATCCTTCTTCCAAAGTCCCTTAG(+CAGTGT)GTACCAAAAACCAACCCAACAGGTTGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240605175417_zf7l AGAGGATACGTGGCTTGAAGCAGCTGCAGAGG(+CAGTGT)GTACCTTCAGCCTTGAGCCACACTCTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605175416_gj8e TTCTGATTTAAGAGTGTGGCTCAAGGCTGAAG(+CAGTGT)GTACCCTCTGCAGCTGCTTCAAGCCACGTAT NGG 10 8 17 8 24 0 100 5 10
    240605175414_6usp CCCCAGTAAAGAAAAGAGAGCAAAAAACACTG(+CAGTGT)GTACCCGCCTTGCTTTCTCCTTTATACAGGA NGG 10 8 17 8 24 0 100 5 10
    240605175413_n8fh TGGGATCCTGTATAAAGGAGAAAGCAAGGCGG(+CAGTGT)GTACCAGTGTTTTTTGCTCTCTTTTCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240605175411_z9ca CTGGTACCCGCCTTGCTTTCTCCTTTATACAG(+CAGTGT)GATCCCAGCCAAGGGAGTGGTACTAACCATG NGG 10 8 17 8 24 0 100 5 10
    240605175410_y24s GACCACATGGTTAGTACCACTCCCTTGGCTGG(+CAGTGT)GATCCTGTATAAAGGAGAAAGCAAGGCGGGT NGG 10 8 17 8 24 0 100 5 10
    240605175408_0yem ATGCAGCTCAGAGAGCAGAGGCCCATGCTTTG(+CAGTGT)GATCCTTAGCCGAGCTGAGCAAGTCCTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605175407_us21 TTTTAAAAGAGGACTTGCTCAGCTCGGCTAAG(+CAGTGT)GATCCAAAGCATGGGCCTCTGCTCTCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605175405_7ijz CTATAGAGGGAAACTAGGGTTCAGAAGAGGGG(+CAGTGT)GATCCAAAATCATACAGAGTCCCAGAGCAGC NGG 10 8 17 8 24 0 100 5 10