Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175436_17ok CAGTGACCCAGGGCTGTGCCTCGAACACTCTG(+CAGTGT)GATCCGCTTACAGAGACTTTCCCAGCACTGC NGG 10 8 17 8 24 0 100 5 10
    240605175434_mtf0 TGGTAGAACAAGTGTCTTGTGATCAGACACCG(+CAGTGT)GATCCCCACCTCAGGCAAAAACCACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605175432_difd CCGAGTTCCTTGTGGTTTTTGCCTGAGGTGGG(+CAGTGT)GATCCGGTGTCTGATCACAAGACACTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240605175430_imig CTTATAGGTCTGAACGCAGGTCCTGCCACCCG(+CAGTGT)GTACCACCAGCCTCAGTACAGTCACTACGGC NGG 10 8 17 8 24 0 100 5 10
    240605175428_nx2d CTCAAGCCGTAGTGACTGTACTGAGGCTGGTG(+CAGTGT)GTACCGGGTGGCAGGACCTGCGTTCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240605175426_casu CCATGCGGCTCAGCCATGCTGGTCTCTCCGAG(+CAGTGT)GTACCCAGATAGCTGCACCCCTCTGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605175424_9x4e AGCAAGGGGAGCAGAGGGGTGCAGCTATCTGG(+CAGTGT)GTACCTCGGAGAGACCAGCATGGCTGAGCCG NGG 10 8 17 8 24 0 100 5 10
    240605175423_obxd ACTGTCTTCAGACACAGCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGACTTGAGCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240605175422_1gn7 AGTACCCACGGGGTGGCTCAAGTCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGCTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605175420_zrph GGCACATGGCCAACCTGTTGGGTTGGTTTTTG(+CAGTGT)GTACCTAAGGGACTTTGGAAGAAGGATCAAG NGG 10 8 17 8 24 0 100 5 10