Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175348_ra2v TTTTCCTCTGACTGACACTGAAGAGCAAATTG(+CAGTGT)GTACCTGGGCTGCTTTCTATTTGTCTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240605175346_8i9x GGGTGGGTGAGTGCTGGCATGGACGTCTCTAG(+CAGTGT)GATCCTCTACAAACAGGCCCAGTTCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240605175345_9own TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+CAGTGT)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240605175343_n91y GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+CAGTGT)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605175341_r9tj AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+CAGTGT)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10
    240605175340_47xr TGTTTAGACACATTTTACTAGGCACCTCCGTG(+CAGTGT)GATCCTTTACAGTGTTCTTGTGACAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240605175338_szw7 CCACACTAGCTTGTCACAAGAACACTGTAAAG(+CAGTGT)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605175337_xcp0 GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+CAGTGT)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240605175335_z1el GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+CAGTGT)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605175334_7pjo ACACAACTCCCCCACAAAAATCACTGGAGTAG(+CAGTGT)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10