Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175245_4ihn AATTCCAGTCTATTGTGTAACCCACCACCAGG(+CAGTGT)GATCCCTTAGTAAACACCCAATTATGCTATG NGG 10 8 17 8 24 0 100 5 10
    240605175244_ltiv CGAGTGGTCATGCCCTCCTTTTCATTCATCTG(+CAGTGT)GTACCAACACCAGAGGGAGAGCATACAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605175243_ea2v GGGAAGTCCTGTATGCTCTCCCTCTGGTGTTG(+CAGTGT)GTACCAGATGAATGAAAAGGAGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605175241_i6a5 TGTGGAAGAAGCTTGCTGCCTGAGGGCTGAGG(+CAGTGT)GATCCATAGGATCTCAATGAGGCTTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240605175239_5uo9 CATGTAACTGCAAGCCTCATTGAGATCCTATG(+CAGTGT)GATCCCTCAGCCCTCAGGCAGCAAGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605175238_bkdv CATCAAGCAACTCTGAGTCTCTCTGGCTTCTG(+CAGTGT)GATCCTGCTACAATATATGTGGGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240605175237_yarf CCCTTTGTTCCTGCCCACATATATTGTAGCAG(+CAGTGT)GATCCAGAAGCCAGAGAGACTCAGAGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605175235_tbwf ACGGCCAAGCCATCCAAAACTAAGGGAAGTGG(+CAGTGT)GATCCCTAAGAGGTGAACCCTTAAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605175233_u99p TGGGGACAACACTTAAGGGTTCACCTCTTAGG(+CAGTGT)GATCCCACTTCCCTTAGTTTTGGATGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605175232_obp9 TTTATATTCCAATTATGCCAACAAAGGGCAAG(+CAGTGT)GTACCAGGAAAAGCATTAAACTATATTTTTT NGG 10 8 17 8 24 0 100 5 10