Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175217_cgu1 ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+CAGTGT)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605175215_8a41 ATGAAGTGGACACTTTTCTAGACAGATACTAG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAGATTAACG NGG 10 8 17 8 24 0 100 5 10
    240605175214_3t5v TAAATCGTTAATCTGATCCTGATTTAACTTTG(+CAGTGT)GTACCTAGTATCTGTCTAGAAAAGTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240605175213_3ex9 TGCTCCTAGTCACCCATTGGATGCAGCACAGG(+CAGTGT)GTACCCAATGAAGGAGCTAAAGAAAGTACCT NGG 10 8 17 8 24 0 100 5 10
    240605175211_ihmq TCTTTAGGTACTTTCTTTAGCTCCTTCATTGG(+CAGTGT)GTACCCTGTGCTGCATCCAATGGGTGACTAG NGG 10 8 17 8 24 0 100 5 10
    240605175210_86ak CATAGTGAGGAAATGAAATGATGCACTTAATG(+CAGTGT)GTACCATCTCACTGTCAAGATAAAATACCCA NGG 10 8 17 8 24 0 100 5 10
    240605175208_r2ip GCAGCTGGGTATTTTATCTTGACAGTGAGATG(+CAGTGT)GTACCATTAAGTGCATCATTTCATTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240605175207_5pkb ATTGTTGAAAGTCAAACTTATTGTGAGTTAAG(+CAGTGT)GATCCATAAAATTCTTCTGTTGCAGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240605175206_k398 AGTATCCCTAACTGCAACAGAAGAATTTTATG(+CAGTGT)GATCCTTAACTCACAATAAGTTTGACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605175204_zhk1 AATCCACTCAGGACCACACAGTAACACATGTG(+CAGTGT)GATCCTGGCACCCCCACTTCTGCCTCCAACT NGG 10 8 17 8 24 0 100 5 10