Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175230_b3s8 GTGTTAAAAAATATAGTTTAATGCTTTTCCTG(+CAGTGT)GTACCTTGCCCTTTGTTGGCATAATTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605175228_2040 CTGTCAGTATAGTTCAAGTTTACCAGTAGAGG(+CAGTGT)GTACCTGAGAGGAACAACCACCACAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240605175227_dh9j TGAGGCAGCTCTGTGGTGGTTGTTCCTCTCAG(+CAGTGT)GTACCCTCTACTGGTAAACTTGAACTATACT NGG 10 8 17 8 24 0 100 5 10
    240605175226_7sa8 AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+CAGTGT)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240605175225_oadd ATATTAGGATGGCTACTCTAGCTTGTTTCTTG(+CAGTGT)GTACCATTTGCTTGGAAAATTGTTTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240605175223_wber ATGAAATGAACAATTTTCTAGACAGATACCTG(+CAGTGT)GTACCAAAGTTAAATCAGGATCAGATAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605175222_7ir5 TTAGATTTTTATCTGATCCTGATTTAACTTTG(+CAGTGT)GTACCAGGTATCTGTCTAGAAAATTGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605175220_avb1 ATGATCTGAAAATACTTACATATACCTGGAAG(+CAGTGT)GTACCTAAGTCATCCAACTGAATGCCAACTG NGG 10 8 17 8 24 0 100 5 10
    240605175219_9jow ATGCCCAGTTGGCATTCAGTTGGATGACTTAG(+CAGTGT)GTACCTTCCAGGTATATGTAAGTATTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605175218_biza AAAAGGCTGGAAAACAATTTTCCAAGCAAATG(+CAGTGT)GTACCAAGAAACAAGCTAGAGTAGCCATCCT NGG 10 8 17 8 24 0 100 5 10