Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175708_xb92 AATGGAGGGAAGGGGCATCTAGCTTAGACAGG(+CAGTGT)GATCCCAGGGGTCTCAGCTTACAGCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605175706_86t0 CTGGGGCTTATGCTTGGCTGTGGGCCTGGTGG(+CAGTGT)GATCCGGGGCACACATTCTCCTAGTGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240605175704_ygkf GAGGGACCAGCACTAGGAGAATGTGTGCCCCG(+CAGTGT)GATCCCACCAGGCCCACAGCCAAGCATAAGC NGG 10 8 17 8 24 0 100 5 10
    240605175701_afni CTCTTAGTTCAAGGAGGTGATGATGGGTGGGG(+CAGTGT)GATCCGGAGGGCTTCTAAGATGTGCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605175659_bdq4 CGAGATTCCAAGCACATCTTAGAAGCCCTCCG(+CAGTGT)GATCCCCCACCCATCATCACCTCCTTGAACT NGG 10 8 17 8 24 0 100 5 10
    240605175657_tv0i TGTCCGAGTCCATGGAGAGAGGCTGTGCTAGG(+CAGTGT)GTACCCAGCCAAAGACTCGCCTTTGAAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605175656_vmx1 ACCAGAGGCTTCAAAGGCGAGTCTTTGGCTGG(+CAGTGT)GTACCCTAGCACAGCCTCTCTCCATGGACTC NGG 10 8 17 8 24 0 100 5 10
    240605175654_4an5 GGCGTGGCCCCATCTTTGGGAACCTGGTGTGG(+CAGTGT)GATCCAGGCAAATCTGGAAGGCAAAAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605175652_poio CCTATGAACTTTTTGCCTTCCAGATTTGCCTG(+CAGTGT)GATCCCACACCAGGTTCCCAAAGATGGGGCC NGG 10 8 17 8 24 0 100 5 10
    240605175650_mqlu AGACTGCACCTTTGTGATTCCTTTTCTCCCGG(+CAGTGT)GATCCACCTGGGACAGTCAGTCTCAGAAGGG NGG 10 8 17 8 24 0 100 5 10