Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175103_icau GTTGAAACAGTTCTTTTTAATAGCCAACAATG(+CAGTGT)GTACCTGGCCAGAAGAAAATGAGGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605175101_y26q GGAGGGGAAACCCCTCCCACCCACCAAGTTTG(+CAGTGT)GATCCTGGACCTCAGCTTGAACAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240605175059_x2yg CTCACTGGAATACTGTTCAAGCTGAGGTCCAG(+CAGTGT)GATCCAAACTTGGTGGGTGGGAGGGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605175058_5skx AAATGCTATGACTTTAAGGGTCTTGGGGGGAG(+CAGTGT)GATCCATGACCTAAACACAGTCGTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605175057_lseg AAAAAAAAAAAAACGACTGTGTTTAGGTCATG(+CAGTGT)GATCCTCCCCCCAAGACCCTTAAAGTCATAG NGG 10 8 17 8 24 0 100 5 10
    240605175055_yacz AATAAAAGTAAGAAAATATGCTCAATGCTGAG(+CAGTGT)GTACCTTAAAAAAACAAAAGTTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605175054_n7yc TAAAATATTTTATAAACTTTTGTTTTTTTAAG(+CAGTGT)GTACCTCAGCATTGAGCATATTTTCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240605175053_6ypn TGCTCAACCTTATTTATATCACTGACTGGCTG(+CAGTGT)GATCCAAAGTCACACCTCCACATATTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605175051_gnpp TTTATTCACTAATATGTGGAGGTGTGACTTTG(+CAGTGT)GATCCAGCCAGTCAGTGATATAAATAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605175050_4vmo ACTGTGGGTGCTTGAGGAGGCCAGAGGCATAG(+CAGTGT)GATCCCTCTGGAGCATGAATTACAAGTGGCT NGG 10 8 17 8 24 0 100 5 10