Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175016_f91e ATCTTAATCTTTATCTGAAAGACTGCTATGGG(+CAGTGT)GATCCCACAGGAAGTCAAGAGAAAAAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605175015_32jk AGAAATTCAGGGCTGAAGAATAGACCCAGGCG(+CAGTGT)GATCCAGGCACAGCCAGATCCACCCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605175013_wqm7 TCTTAGCTACAGGGTGGATCTGGCTGTGCCTG(+CAGTGT)GATCCGCCTGGGTCTATTCTTCAGCCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240605175012_7brf TTGAAGTCCCACTGTTGGCAGTTTTGGCAGTG(+CAGTGT)GTACCTAACTCATCGTGTTTGCATTCTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605175010_uf6l AACTTAACAAGAATGCAAACACGATGAGTTAG(+CAGTGT)GTACCACTGCCAAAACTGCCAACAGTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605175009_f8zg AGAAGTCAATGCTGTTCAGATGAAGTCTCTAG(+CAGTGT)GTACCAAACCAACATGTTCTCTTTTATCAAT NGG 10 8 17 8 24 0 100 5 10
    240605175008_yg1a CGAGCATTGATAAAAGAGAACATGTTGGTTTG(+CAGTGT)GTACCTAGAGACTTCATCTGAACAGCATTGA NGG 10 8 17 8 24 0 100 5 10
    240605175007_zwtd GCAACTGGAGGGGCCGTGAGGAGGAGGAGCTG(+CAGTGT)GTACCTTGCTAGGCGGAAGCAGGCACTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605175006_pytc CTCTGTCACAGTGCCTGCTTCCGCCTAGCAAG(+CAGTGT)GTACCAGCTCCTCCTCCTCACGGCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240605175004_ku51 TCTTCAGACAATGTTGCTTTGCTGCCTGTTTG(+CAGTGT)GATCCTTGTACATTTTACTTTACAAGAGAGA NGG 10 8 17 8 24 0 100 5 10