Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605175032_k1f0 TAGTGTATAAAAAGGTGCCCACTATTATGCAG(+CAGTGT)GTACCTTTGCTACATAAAGTATTTACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240605175031_i8i4 ACAAAAAACCAACTAAGAAAGTTAATGATAAG(+CAGTGT)GTACCATTGATGGTGAAATGTTTGGTCAAAC NGG 10 8 17 8 24 0 100 5 10
    240605175030_ah9q ATTATGTTTGACCAAACATTTCACCATCAATG(+CAGTGT)GTACCTTATCATTAACTTTCTTAGTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605175028_9qwd CATAGCACCGCTATATGCTGGCATCAGAGTTG(+CAGTGT)GTACCCACGCCCCGTCCATTAGTCACAGCAC NGG 10 8 17 8 24 0 100 5 10
    240605175027_i7to ACTCAGTGCTGTGACTAATGGACGGGGCGTGG(+CAGTGT)GTACCAACTCTGATGCCAGCATATAGCGGTG NGG 10 8 17 8 24 0 100 5 10
    240605175026_54s1 TCCCTGTTCACCTCCTTACACAGAAAGATGTG(+CAGTGT)GTACCCAGGGTAGAATCACAGCTCCATACAG NGG 10 8 17 8 24 0 100 5 10
    240605175023_3zr8 GTACACTGTATGGAGCTGTGATTCTACCCTGG(+CAGTGT)GTACCACATCTTTCTGTGTAAGGAGGTGAAC NGG 10 8 17 8 24 0 100 5 10
    240605175021_xq7z AGAGATCGCCTGCCTTGTGTCATGAGTGCTGG(+CAGTGT)GATCCATAAGCCACCATGCTTGGCTGATGTT NGG 10 8 17 8 24 0 100 5 10
    240605175020_zw2i AGGGAAACATCAGCCAAGCATGGTGGCTTATG(+CAGTGT)GATCCCAGCACTCATGACACAAGGCAGGCGA NGG 10 8 17 8 24 0 100 5 10
    240605175018_gg5z AAACTACATTTTTTTCTCTTGACTTCCTGTGG(+CAGTGT)GATCCCCATAGCAGTCTTTCAGATAAAGATT NGG 10 8 17 8 24 0 100 5 10