Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605174947_5y2y GGCCCTATCTTTAAACAGTTTTTGTGCACCAG(+CAGTGT)GATCCCTCCCATGACACTTATGGTATCATTA NGG 10 8 17 8 24 0 100 5 10
    240605174946_2wff TCTTAAGTGAGCACTAACTTGTTACAGTGCTG(+CAGTGT)GATCCAGCCAGCCCCAGAGGCCCAATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240605174945_z2qo AGTTAATGAGATTGGGCCTCTGGGGCTGGCTG(+CAGTGT)GATCCAGCACTGTAACAAGTTAGTGCTCACT NGG 10 8 17 8 24 0 100 5 10
    240605174943_8tlt AAAAGGACACATAGTATATAACTCCACTGTTG(+CAGTGT)GTACCAACAATATTTACTGCAGAAAACCACG NGG 10 8 17 8 24 0 100 5 10
    240605174942_4jdg GTTTCCGTGGTTTTCTGCAGTAAATATTGTTG(+CAGTGT)GTACCAACAGTGGAGTTATATACTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240605174940_l8w3 AAGTAGAAAATCATCTTTAAAAGACAGCCAAG(+CAGTGT)GTACCATCACATCTCCCTGAAAGTAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240605174939_7680 CTGTTCTGTCTTACTTTCAGGGAGATGTGATG(+CAGTGT)GTACCTTGGCTGTCTTTTAAAGATGATTTTC NGG 10 8 17 8 24 0 100 5 10
    240605174938_1ym4 ATTGATTCCTACTTTATTATCTTAAAGACTAG(+CAGTGT)GATCCTGTTATTTTTCTTTAAATGTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605174937_vmw4 AGTGCCTGTGAACATTTAAAGAAAAATAACAG(+CAGTGT)GATCCTAGTCTTTAAGATAATAAAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605174936_wql3 AAGGCCTACCTCCAGGGATGCAGCTCCTACAG(+CAGTGT)GATCCCAGAATGAAGTTGACTCGAATATTAC NGG 10 8 17 8 24 0 100 5 10