Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170222_wlsn AGATGTGATCTTGCCAAAGCCATTAGACCTTG(+AGGACA)GTACCCAGGCCCTCCTATAATGAGCTGATTG NGG 10 8 17 8 24 0 100 5 10
    240613170219_27z1 GCAGACAATCAGCTCATTATAGGAGGGCCTGG(+AGGACA)GTACCAAGGTCTAATGGCTTTGGCAAGATCA NGG 10 8 17 8 24 0 100 5 10
    240613170218_uesb TCTGTGTACCATTTGAATGCCTGGCACTGAGG(+AGGACA)GATCCCCTGAAATAAGGCTATAGAGGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613170216_s832 GGCACACAACCCTCTATAGCCTTATTTCAGGG(+AGGACA)GATCCCTCAGTGCCAGGCATTCAAATGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613170214_m4mh GTTAGGAAACACATGGCAGCCTAAGGCAAGAG(+AGGACA)GATCCCAAGGTTAAGTCCTGTTTCAGACACC NGG 10 8 17 8 24 0 100 5 10
    240613170212_s3cl GACAGGGTGTCTGAAACAGGACTTAACCTTGG(+AGGACA)GATCCTCTTGCCTTAGGCTGCCATGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613170211_bc44 CTTTTCATTGAGAGGTTGTAAATGGTGCTGTG(+AGGACA)GTACCTGATAAGAAAGTCAGTCTCTGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240613170209_ajxq GGTCCTACCACAGAGACTGACTTTCTTATCAG(+AGGACA)GTACCACAGCACCATTTACAACCTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240613170207_4nzi TTTCATTTTGAAGTCAGCCACACTGGGCCTGG(+AGGACA)GATCCTGGTCAGCAGACTCAGGGCGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613170206_qwky CCCTGTGCAGACGCCCTGAGTCTGCTGACCAG(+AGGACA)GATCCCAGGCCCAGTGTGGCTGACTTCAAAA NGG 10 8 17 8 24 0 100 5 10