Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170329_0nsk TCCATCTCTAAGCATCGAAGCAAGGAGCCCTG(+AGGACA)GATCCCCAAGAGGTTCCTGAGTGCAGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613170327_ki5f AGTGCTGGAACTGCACTCAGGAACCTCTTGGG(+AGGACA)GATCCAGGGCTCCTTGCTTCGATGCTTAGAG NGG 10 8 17 8 24 0 100 5 10
    240613170326_b7ip CGATGGAGCCTGATGACATTAGAGCCCTTTTG(+AGGACA)GTACCAGAAGGCAGCTGGAGCAGGGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613170324_ez4u TGGCTCCCAGGCCCTGCTCCAGCTGCCTTCTG(+AGGACA)GTACCAAAAGGGCTCTAATGTCATCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240613170323_6j21 ACTGGAAGGGAAAAGAAGGGATCTGGGTGCCG(+AGGACA)GATCCTCAGGCGCAGGTGGTGACCACAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613170321_v24n CCTGGCCCCTGTGGTCACCACCTGCGCCTGAG(+AGGACA)GATCCGGCACCCAGATCCCTTCTTTTCCCTT NGG 10 8 17 8 24 0 100 5 10
    240613170319_dpab CCCCAGAAACAAATGCAACCTACAATTGTGTG(+AGGACA)GTACCGGGACCTCGAGTTCTGATCTGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240613170317_962q CTGACAAGACCAGATCAGAACTCGAGGTCCCG(+AGGACA)GTACCACACAATTGTAGGTTGCATTTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613170316_plzb ACAGTATCTAAAGGTAGTTATTTCTCCTTCTG(+AGGACA)GATCCGGTGCAGCATACCTATGATTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613170314_7pzi TCTAGAGCTGGAATCATAGGTATGCTGCACCG(+AGGACA)GATCCAGAAGGAGAAATAACTACCTTTAGAT NGG 10 8 17 8 24 0 100 5 10