Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170348_ah5p CCCTGAGTGGGGTGGGTGGGGCTAGGAAGGAG(+AGGACA)GTACCCCACAGCTTTCTGCATCTATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240613170345_1um8 CTGGCCTCCATATAGATGCAGAAAGCTGTGGG(+AGGACA)GTACCTCCTTCCTAGCCCCACCCACCCCACT NGG 10 8 17 8 24 0 100 5 10
    240613170343_rqun GTGTCAAATCCTCTTCTCTACATAGTTCCCAG(+AGGACA)GATCCCAGATCTCTTGGCCCATCCCATGAGG NGG 10 8 17 8 24 0 100 5 10
    240613170341_0hx5 AGGTACCTCATGGGATGGGCCAAGAGATCTGG(+AGGACA)GATCCTGGGAACTATGTAGAGAAGAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240613170339_ci7s ATACTTAGGGTCACCAGGGGGAGCACTCTCCG(+AGGACA)GTACCCTTCACCTGGGTTTTAAATGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613170338_zi8g CAGAGAGAAGCCATTTAAAACCCAGGTGAAGG(+AGGACA)GTACCGGAGAGTGCTCCCCCTGGTGACCCTA NGG 10 8 17 8 24 0 100 5 10
    240613170336_2ehd TTAAATGGCTTCTCTCTGTCTGGATCACCTAG(+AGGACA)GATCCAGTTTTAGTGAACCCTCTTCCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613170335_7f59 GTATCTCTTTGGAAGAGGGTTCACTAAAACTG(+AGGACA)GATCCTAGGTGATCCAGACAGAGAGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613170332_3x59 CAGCCCTAGGACCAAGAACAACATTGCCTTGG(+AGGACA)GTACCTTTGTAGTGAGTCACGGCTCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613170331_4s4z GCTGTACTCCAGAGCCGTGACTCACTACAAAG(+AGGACA)GTACCCAAGGCAATGTTGTTCTTGGTCCTAG NGG 10 8 17 8 24 0 100 5 10