Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170422_5v3m TTCTTGAGGTGTTGTGTGTTTGTTTAGATGGG(+AGGACA)GTACCTGGGATACTCCACATGACCATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613170420_jn4x CTGGCTTCTGATGGTCATGTGGAGTATCCCAG(+AGGACA)GTACCCCATCTAAACAAACACACAACACCTC NGG 10 8 17 8 24 0 100 5 10
    240613170419_zvop TGGGGTGAATAGTACTTGTTGCTTTTATAGAG(+AGGACA)GATCCAAGCACTGACATGGAGCCTCACAGTC NGG 10 8 17 8 24 0 100 5 10
    240613170417_a913 AAGATGACTGTGAGGCTCCATGTCAGTGCTTG(+AGGACA)GATCCTCTATAAAAGCAACAAGTACTATTCA NGG 10 8 17 8 24 0 100 5 10
    240613170415_4rqj CAGCCCAACTGGACCAGAGCTCAGGGGTCCTG(+AGGACA)GTACCTTTCTTCCCATCATAGACAACATTAT NGG 10 8 17 8 24 0 100 5 10
    240613170414_ds9d TTTTAATAATGTTGTCTATGATGGGAAGAAAG(+AGGACA)GTACCAGGACCCCTGAGCTCTGGTCCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613170413_fp0v GGCATGAAGGAGGAAAGAGCATCTTGGAGCTG(+AGGACA)GTACCCAACCAAGGCTTGCTTGTTCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613170411_1owm GCTTCTTTGGGGAACAAGCAAGCCTTGGTTGG(+AGGACA)GTACCAGCTCCAAGATGCTCTTTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613170409_bkvs GAGGCTTCAGCGAGCATCGATGTCTGCCTCAG(+AGGACA)GATCCTTACATCCCTGCATAGCATCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613170407_6jz2 GTGTACACACTGATGCTATGCAGGGATGTAAG(+AGGACA)GATCCTGAGGCAGACATCGATGCTCGCTGAA NGG 10 8 17 8 24 0 100 5 10