Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170511_2ak9 GGCAAAGAACCCTGACAGTGGCAAACAAGCAG(+AGGACA)GATCCCCCAGGCGCTGGCCCTAGAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613170509_h3ib TATCAATTAGGCTCTAGGGCCAGCGCCTGGGG(+AGGACA)GATCCTGCTTGTTTGCCACTGTCAGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613170508_xsne CACTGACAAGTGATATTAGCCATAAAGTGCAG(+AGGACA)GTACCATGCCACACTCCACAGACCAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613170507_enu1 TTTCACCTCCTTGGTCTGTGGAGTGTGGCATG(+AGGACA)GTACCTGCACTTTATGGCTAATATCACTTGT NGG 10 8 17 8 24 0 100 5 10
    240613170504_aly8 TGTTGCAGGGCTATAGGAAGACAAAGCCTGGG(+AGGACA)GTACCCGCATCTGGTTTGGAACCCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613170503_s0y2 CAACAAAACTCAGGGTTCCAAACCAGATGCGG(+AGGACA)GTACCCCAGGCTTTGTCTTCCTATAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613170501_yauo TTTTCTGTTTCACATCTTGTTTTTAAAAATAG(+AGGACA)GATCCAAAACATCTCAAATCATCTCCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613170500_3gj9 ATTCTGTTAAGGAGATGATTTGAGATGTTTTG(+AGGACA)GATCCTATTTTTAAAAACAAGATGTGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613170459_36p2 GTTTGTTATCATAGTTGGTCAATGCCTGGCAG(+AGGACA)GTACCAGCATTTTGTCACTTGGGTCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240613170457_8nig TCCCTTGGTTTGACCCAAGTGACAAAATGCTG(+AGGACA)GTACCTGCCAGGCATTGACCAACTATGATAA NGG 10 8 17 8 24 0 100 5 10