Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170405_kura GGTTACCTACCCACCACCCCTCCAGGCACCAG(+AGGACA)GATCCCCTATTTAGCTTCCTGTAACGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613170403_x36o GCTGTGGGGGCGTTACAGGAAGCTAAATAGGG(+AGGACA)GATCCTGGTGCCTGGAGGGGTGGTGGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613170400_ox33 GCTTTGGTATAGTGAATGTCTCCCTGTGCAGG(+AGGACA)GTACCACGCCATGTGCCATTTAGGCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240613170359_lnkw GTCTGGCTTGTGCCTAAATGGCACATGGCGTG(+AGGACA)GTACCCTGCACAGGGAGACATTCACTATACC NGG 10 8 17 8 24 0 100 5 10
    240613170357_g2ct ATATATCCAGTCAAGGAGCAATCACACTTTGG(+AGGACA)GATCCGCATCTCAAAGATAGGGTGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613170356_5sdf CATCTCCTCTCTCACCCTATCTTTGAGATGCG(+AGGACA)GATCCCAAAGTGTGATTGCTCCTTGACTGGA NGG 10 8 17 8 24 0 100 5 10
    240613170354_1lnc AAAGGAAAGGTGTCTCTCCTACGGGCCAGGGG(+AGGACA)GATCCTATCACAAAAGAATAAAGCAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613170353_g39p TCCAATCAGGCTGCTTTATTCTTTTGTGATAG(+AGGACA)GATCCCCCTGGCCCGTAGGAGAGACACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613170351_gw0j AGGATCCCAGATCTCTTGGCCCATCCCATGAG(+AGGACA)GTACCTGACTGTCTACCAGCACTGTCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613170350_wmkp GGCTACCAGAGACAGTGCTGGTAGACAGTCAG(+AGGACA)GTACCTCATGGGATGGGCCAAGAGATCTGGG NGG 10 8 17 8 24 0 100 5 10