Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170437_y5z4 CATAACAGTTCATATTGACCTTTGATTTAATG(+AGGACA)GATCCTACATAAAGCTTTGTGGAATTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613170436_55i0 AATCTTAAGAAATTCCACAAAGCTTTATGTAG(+AGGACA)GATCCATTAAATCAAAGGTCAATATGAACTG NGG 10 8 17 8 24 0 100 5 10
    240613170434_tkbb CTGTGAAGCCCCTGTTATATACTACCTGGTGG(+AGGACA)GTACCTTGCCAACTTTTCCTGTGAAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240613170433_y26y GTTGATCTGGTTTCACAGGAAAAGTTGGCAAG(+AGGACA)GTACCCACCAGGTAGTATATAACAGGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613170431_8liz TGCTCTAACCCTATGTGAGGTTTCAGAAGGGG(+AGGACA)GATCCTCGCTAGGATACATCCAGCCACATCC NGG 10 8 17 8 24 0 100 5 10
    240613170430_0o8c TAAGTGGATGTGGCTGGATGTATCCTAGCGAG(+AGGACA)GATCCCCCTTCTGAAACCTCACATAGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240613170429_th2n CATTATCAGGCAAAAGCAACTGTTTGGTCAAG(+AGGACA)GATCCTCATATAAAAATCACATTTCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240613170427_rneg TCCGATGTGAAGAAATGTGATTTTTATATGAG(+AGGACA)GATCCTTGACCAAACAGTTGCTTTTGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613170426_g6mi AGGCTGGGGCATTGTCTGACAGCATTTGTTTG(+AGGACA)GATCCACATAGTGCTGCTGCTGCTGCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613170425_znz3 TGCAGCAACAGCAGCAGCAGCAGCACTATGTG(+AGGACA)GATCCAAACAAATGCTGTCAGACAATGCCCC NGG 10 8 17 8 24 0 100 5 10