Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170317_962q CTGACAAGACCAGATCAGAACTCGAGGTCCCG(+AGGACA)GTACCACACAATTGTAGGTTGCATTTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613170316_plzb ACAGTATCTAAAGGTAGTTATTTCTCCTTCTG(+AGGACA)GATCCGGTGCAGCATACCTATGATTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613170314_7pzi TCTAGAGCTGGAATCATAGGTATGCTGCACCG(+AGGACA)GATCCAGAAGGAGAAATAACTACCTTTAGAT NGG 10 8 17 8 24 0 100 5 10
    240613170312_n79r TTTGATAGTAAGATTATAAGGTGGCTCCAGAG(+AGGACA)GTACCTTGGATAGGACTGCGCCCTGATAAGA NGG 10 8 17 8 24 0 100 5 10
    240613170311_tkxf TTTCATCTTATCAGGGCGCAGTCCTATCCAAG(+AGGACA)GTACCTCTGGAGCCACCTTATAATCTTACTA NGG 10 8 17 8 24 0 100 5 10
    240613170309_yb70 GCGCCAAGTGGAACGTGGAGAGTGGAGGCCTG(+AGGACA)GTACCTGAGGTTTTAGTGGTAACCAAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613170307_35eg TAGATAGTCCTTGGTTACCACTAAAACCTCAG(+AGGACA)GTACCAGGCCTCCACTCTCCACGTTCCACTT NGG 10 8 17 8 24 0 100 5 10
    240613170305_yji6 GGGGACATTGTTAAATAGGGAAGGTGATGGTG(+AGGACA)GATCCTGACTGCTCCTACCCTGTGACCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613170304_r60v ATGACTAAAGGTCACAGGGTAGGAGCAGTCAG(+AGGACA)GATCCACCATCACCTTCCCTATTTAACAATG NGG 10 8 17 8 24 0 100 5 10
    240613170302_dq8j ACAAGCACCCCTAAACCACCTCTGCCTACAGG(+AGGACA)GATCCCCAGGACCCCAATGGGCATCAGCCTT NGG 10 8 17 8 24 0 100 5 10