Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170120_42gb GTGTTTTCTGTCCAAAACTCTGAAATAATAAG(+AGGACA)GATCCCTTAGAGTATGGCAAAACCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613170118_it20 ATTATGTTTGGGGGTTTTGCCATACTCTAAGG(+AGGACA)GATCCTTATTATTTCAGAGTTTTGGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240613170117_nrp6 GCCTAGTGCACATCTATGAGCCCATTATTCGG(+AGGACA)GATCCAGAGGCAGAAGGATCAGAGTTCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613170115_57f2 GATAACCTTGAACTCTGATCCTTCTGCCTCTG(+AGGACA)GATCCCGAATAATGGGCTCATAGATGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240613170113_e7yf AGCACCCTGTGAACAAAGAGCTGGTCACAGTG(+AGGACA)GTACCCGAGTTTCTGTGACTGAGTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613170112_vyao GCTTTCAGACAGACTCAGTCACAGAAACTCGG(+AGGACA)GTACCACTGTGACCAGCTCTTTGTTCACAGG NGG 10 8 17 8 24 0 100 5 10
    240613170110_693e TCTGCCCATTTAAAATGATAATGAACTATGGG(+AGGACA)GTACCAAGCATAGTTAATATGTAAATACAAG NGG 10 8 17 8 24 0 100 5 10
    240613170108_wg0l ATGTACTTGTATTTACATATTAACTATGCTTG(+AGGACA)GTACCCCATAGTTCATTATCATTTTAAATGG NGG 10 8 17 8 24 0 100 5 10
    240613170107_l5vf TGTGACACTTTGCAACACAATCCCAACATTAG(+AGGACA)GTACCAGTTAAAAGATGGCTTTCCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240613170105_14uh GGGCAGGAGTTGGGAAAGCCATCTTTTAACTG(+AGGACA)GTACCTAATGTTGGGATTGTGTTGCAAAGTG NGG 10 8 17 8 24 0 100 5 10