Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613170013_uoos GTTCTGAAGTAGATGGTTGCCATGAACTCCCG(+AGGACA)GATCCAGATAGTCTCATTCATGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613170012_ytq6 GGGATCACCTCCATCATGAATGAGACTATCTG(+AGGACA)GATCCGGGAGTTCATGGCAACCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240613170011_cbna AATGAAAAGCAGCTAACTACAGAAGCAACATG(+AGGACA)GATCCTGCTCTGATTACCCATCGTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613170010_fams TCAGCTCTAGGGACGATGGGTAATCAGAGCAG(+AGGACA)GATCCATGTTGCTTCTGTAGTTAGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613170008_83jh CAGGATGAGAACAGGCTGGGATGAACTCAGAG(+AGGACA)GTACCAGATGAGCTTGATCTTGAAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613170007_z5f6 TGAGTCAGACTCTTCAAGATCAAGCTCATCTG(+AGGACA)GTACCTCTGAGTTCATCCCAGCCTGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613170006_hhvb GTTTCACAACGCTGAAAATAAGGTGGCAGGTG(+AGGACA)GTACCCTTCCTTCCCAGAAGGAAAGAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240613170004_nk17 TCTTCTTCTCTCTTTCCTTCTGGGAAGGAAGG(+AGGACA)GTACCACCTGCCACCTTATTTTCAGCGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613170003_ryy5 AGCCACCTTATGGATGCTGAGAGTGTAAGCTG(+AGGACA)GATCCTCTACAAGAGCAGCCAGAGCTCTTAT NGG 10 8 17 8 24 0 100 5 10
    240613170001_28n7 AATGGATAAGAGCTCTGGCTGCTCTTGTAGAG(+AGGACA)GATCCAGCTTACACTCTCAGCATCCATAAGG NGG 10 8 17 8 24 0 100 5 10