Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165925_oy1o GCACTTGCCACCAAGTCTAGAGACTTGAGTTG(+AGGACA)GATCCCCAGGACTAACTTTGTTGGAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613165922_618s AGTCAGTTCTTTCCAACAAAGTTAGTCCTGGG(+AGGACA)GATCCAACTCAAGTCTCTAGACTTGGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613165921_pzxz GGCAGCCTCCACACCTGCTGTGCTTAGGACAG(+AGGACA)GATCCTTTGATCACAAGGCAGAACCCTAACC NGG 10 8 17 8 24 0 100 5 10
    240613165920_e4fw ACCGGGGTTAGGGTTCTGCCTTGTGATCAAAG(+AGGACA)GATCCTGTCCTAAGCACAGCAGGTGTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613165919_zszo CCGCTAGCCAAGAAAACACTAGAGAGCTGCAG(+AGGACA)GTACCTGCCACTTGGTCCTGGGAGGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613165917_6brm GTGGCCTTTGACCTCCCAGGACCAAGTGGCAG(+AGGACA)GTACCTGCAGCTCTCTAGTGTTTTCTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613165915_3z88 CTCCCACTCCTTGGTCTGTGTGTTTCTCCAGG(+AGGACA)GATCCCTGTCTGTGACTCTCCGCTGTCGGGG NGG 10 8 17 8 24 0 100 5 10
    240613165913_peiw AACCCCCCCGACAGCGGAGAGTCACAGACAGG(+AGGACA)GATCCCTGGAGAAACACACAGACCAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240613165911_dlmz TATGCATGGAGTCAGTGGAACCTTTCCCACGG(+AGGACA)GATCCTATCTTTGTCTGCGCTAAGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613165910_mfxb GGGCTTCCAGCCCTTAGCGCAGACAAAGATAG(+AGGACA)GATCCCGTGGGAAAGGTTCCACTGACTCCAT NGG 10 8 17 8 24 0 100 5 10