Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165820_mxy4 TTTGTAGTCTCGGGTGAATTAGGCAGGACCCG(+AGGACA)GATCCAAGGGAGACCTGGAGCACAACCGACG NGG 10 8 17 8 24 0 100 5 10
    240613165817_41lg ATCCGCGTCGGTTGTGCTCCAGGTCTCCCTTG(+AGGACA)GATCCGGGTCCTGCCTAATTCACCCGAGACT NGG 10 8 17 8 24 0 100 5 10
    240613165816_bczc TCCTGTTGAATGACCCTCATTGCCTTGGTGTG(+AGGACA)GATCCCAGCCCTCACCTCCTGCAACACTAAT NGG 10 8 17 8 24 0 100 5 10
    240613165814_sstc CTTAAATTAGTGTTGCAGGAGGTGAGGGCTGG(+AGGACA)GATCCACACCAAGGCAATGAGGGTCATTCAA NGG 10 8 17 8 24 0 100 5 10
    240613165812_ablc TTATTAAAATTTCACACAAACACAAGCACAGG(+AGGACA)GTACCAGAGCTCTCTTCCTGGATACCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240613165811_jggk AAAGCTACCTGGTATCCAGGAAGAGAGCTCTG(+AGGACA)GTACCCTGTGCTTGTGTTTGTGTGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240613165810_p0np CTTTTGATGTGGATAATGTGTCAAACTATGTG(+AGGACA)GATCCCAATGTCAGGATGAAAAAAAGTATCC NGG 10 8 17 8 24 0 100 5 10
    240613165808_3mzm AGCATGGATACTTTTTTTCATCCTGACATTGG(+AGGACA)GATCCACATAGTTTGACACATTATCCACATC NGG 10 8 17 8 24 0 100 5 10
    240613165806_tnh9 ATGAGATGAAATATTTGCTTCGCACACGCAGG(+AGGACA)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613165805_s4wx AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+AGGACA)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10