Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165746_cekr CACCATATCCTCCTGTTGTGACTGGTGTGATG(+AGGACA)GTACCTCAGCCAGGTCCTTCAGCAGTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613165745_c3gg GGCGTGGCAGACTGCTGAAGGACCTGGCTGAG(+AGGACA)GTACCATCACACCAGTCACAACAGGAGGATA NGG 10 8 17 8 24 0 100 5 10
    240613165744_02uu TCAAGTCCTTGGGGTTTTGTTATTTTGCACTG(+AGGACA)GATCCACCTGTTGTAAATAGTCTTAAATACA NGG 10 8 17 8 24 0 100 5 10
    240613165742_n5tt AAATGTGTATTTAAGACTATTTACAACAGGTG(+AGGACA)GATCCAGTGCAAAATAACAAAACCCCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613165740_jys8 CCAAGTGCAGAGCCAGTGTTGGACCACCTCAG(+AGGACA)GATCCCCTTGTGGAGACTGCCTGGTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240613165738_gs9c ATTCAGAGGTCACCAGGCAGTCTCCACAAGGG(+AGGACA)GATCCTGAGGTGGTCCAACACTGGCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613165736_4ne6 GCCGCAGGCGCCCAAGCGGACTCACCCGGCAG(+AGGACA)GATCCGAGTCCTAGGCGCGGCACAGTGGCGG NGG 10 8 17 8 24 0 100 5 10
    240613165735_uh83 CACCGCCGCCACTGTGCCGCGCCTAGGACTCG(+AGGACA)GATCCTGCCGGGTGAGTCCGCTTGGGCGCCT NGG 10 8 17 8 24 0 100 5 10
    240613165733_5bw7 TAAAATAATGAGACAGTTTTTTGTTATTGGTG(+AGGACA)GATCCAGGAGCTGTTTAGAAACTATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613165731_9t3r GGGGAAAATACATAGTTTCTAAACAGCTCCTG(+AGGACA)GATCCACCAATAACAAAAAACTGTCTCATTA NGG 10 8 17 8 24 0 100 5 10