Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165729_dw70 TGGGGCCCAGCTGCGGCTGGCTCCCGGCCTGG(+AGGACA)GTACCAGAAAGAGACCTTCAGCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613165728_xo04 CCAGCCTGGGTTGAGCTGAAGGTCTCTTTCTG(+AGGACA)GTACCCAGGCCGGGAGCCAGCCGCAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613165726_n80t TCTGTTGTTACTAATAACAGACTTTATCATGG(+AGGACA)GTACCAGCCATAGCGAGTTAATAAGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240613165725_e4dt TGGATATGTCACTTATTAACTCGCTATGGCTG(+AGGACA)GTACCCATGATAAAGTCTGTTATTAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240613165723_zjky TTCGACATTTTAACCTGTTCTGCCTGACATGG(+AGGACA)GATCCTCCATCCCAGCTCCTTCCCCAACACT NGG 10 8 17 8 24 0 100 5 10
    240613165722_cqua AGCTCAGTGTTGGGGAAGGAGCTGGGATGGAG(+AGGACA)GATCCCATGTCAGGCAGAACAGGTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240613165720_aeek TTGATGCCTTCTTTTAAAGTACCCTGCTCCTG(+AGGACA)GTACCCAGTTAATCCCCTCTCCCAGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240613165718_riwx CCGACTTTATACTGGGAGAGGGGATTAACTGG(+AGGACA)GTACCAGGAGCAGGGTACTTTAAAAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613165717_r5b5 GTGCAACCTGTTACCTTACACAGTGCCTCATG(+AGGACA)GTACCCTGGGAGTGTGCGGGCATCATTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613165714_4zfm ACGGTTGGGAATGATGCCCGCACACTCCCAGG(+AGGACA)GTACCATGAGGCACTGTGTAAGGTAACAGGT NGG 10 8 17 8 24 0 100 5 10