Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165630_w3dg AAGAAGGAGGAGGAGGGATGAGTGGTCTAGTG(+AGGACA)GTACCCTCGTGCCAACAGGGTTCGTGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240613165629_u9le TTTGCACCACCACGAACCCTGTTGGCACGAGG(+AGGACA)GTACCACTAGACCACTCATCCCTCCTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613165628_rvwp TCATAAAAAATAACTCATAGGTACAAGTAATG(+AGGACA)GTACCATATGAGTATGAGTGTAGCCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613165627_ee5o GCTCAGAGAAAGGCTACACTCATACTCATATG(+AGGACA)GTACCATTACTTGTACCTATGAGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240613165625_wqu0 CAGAAAATGTTCTTAGGGCATCATGTCACAAG(+AGGACA)GATCCTTGTAACATATTGGCTCAAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613165624_v95a AGTCTAGAAAATTTGAGCCAATATGTTACAAG(+AGGACA)GATCCTTGTGACATGATGCCCTAAGAACATT NGG 10 8 17 8 24 0 100 5 10
    240613165623_uwmm GGTGAGTACCACTATGCTTGGCTTCCCTTTTG(+AGGACA)GATCCTCTCCCCCTTTCAGCAAAAAAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613165622_biji AGGGTAACTTTTTTTTGCTGAAAGGGGGAGAG(+AGGACA)GATCCAAAAGGGAAGCCAAGCATAGTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613165620_alca GCTGCATGAATGAAATGATGTGATGATGGAGG(+AGGACA)GATCCTGCTTTCTAAACTTTATGAACTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613165618_ziya TGGCTTGAGAGTTCATAAAGTTTAGAAAGCAG(+AGGACA)GATCCCTCCATCATCACATCATTTCATTCAT NGG 10 8 17 8 24 0 100 5 10