Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165534_v2cs CAGTTTAGTTACCCCACAAAGGGCAATATATG(+AGGACA)GATCCTTAGGTTAGCCTTGTTTTATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613165532_nt7d AGTCCTGTTTCATAAAACAAGGCTAACCTAAG(+AGGACA)GATCCATATATTGCCCTTTGTGGGGTAACTA NGG 10 8 17 8 24 0 100 5 10
    240613165531_czgv TCACATGTACATGGGCACTGGCAGGCACAGAG(+AGGACA)GTACCTATTGCATGCACACCAAGCAACAAGT NGG 10 8 17 8 24 0 100 5 10
    240613165530_crf6 TGCACACTTGTTGCTTGGTGTGCATGCAATAG(+AGGACA)GTACCTCTGTGCCTGCCAGTGCCCATGTACA NGG 10 8 17 8 24 0 100 5 10
    240613165528_tni8 TGCTCCTGTGTAATCATTATCCTTTGTTGTAG(+AGGACA)GATCCTTAAGAAGATAATCAAAATATCCTAA NGG 10 8 17 8 24 0 100 5 10
    240613165527_xqq7 TTCCTTTAGGATATTTTGATTATCTTCTTAAG(+AGGACA)GATCCTACAACAAAGGATAATGATTACACAG NGG 10 8 17 8 24 0 100 5 10
    240613165526_71na CCTATGATCAAATTCTGGAGTCTTTGTTCTCG(+AGGACA)GTACCTCCTAAAAAGGAAAGTTGATTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613165525_ttd5 GTTACACAAGAATCAACTTTCCTTTTTAGGAG(+AGGACA)GTACCGAGAACAAAGACTCCAGAATTTGATC NGG 10 8 17 8 24 0 100 5 10
    240613165523_2a8x GTTCTAGATGCTTGAGATCAGTAAGCTAAGCG(+AGGACA)GTACCCAAGGGGGCATCCCCCTCTCTGCGGA NGG 10 8 17 8 24 0 100 5 10
    240613165520_wko6 CCTTCTCCGCAGAGAGGGGGATGCCCCCTTGG(+AGGACA)GTACCGCTTAGCTTACTGATCTCAAGCATCT NGG 10 8 17 8 24 0 100 5 10