Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165447_vurt AGGAGTTAGTTAAATGCTGAAGAGGCTCTCAG(+AGGACA)GTACCCACATCCTGAACCGTGGGGGCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613165445_b95p CCTGGGCTCAGCCCCCACGGTTCAGGATGTGG(+AGGACA)GTACCTGAGAGCCTCTTCAGCATTTAACTAA NGG 10 8 17 8 24 0 100 5 10
    240613165444_um2x CAGGACAGGTTAAAAGGAAACAGCCAAGTGAG(+AGGACA)GTACCTTAGCTGGCAGCAGCGCGAGGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240613165442_xy6n CCTGAACCGTGGGGGCTGAGCCCAGGTGGATG(+AGGACA)GATCCAGTGCAGAGTTACCAACCTCCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240613165440_qgvw TGCCCATCGAGGAGGTTGGTAACTCTGCACTG(+AGGACA)GATCCATCCACCTGGGCTCAGCCCCCACGGT NGG 10 8 17 8 24 0 100 5 10
    240613165439_5v7i GCACTTTAGAGTGGAAAGTTTCTAGTTCAAAG(+AGGACA)GATCCTTTCCTTTCCTGCGAGTGCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613165438_4jvw TCGGGGGAGAAAGCACTCGCAGGAAAGGAAAG(+AGGACA)GATCCTTTGAACTAGAAACTTTCCACTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613165437_p62e AACAAAAACCAGTCTACAATCAATGTGGTAAG(+AGGACA)GTACCTGGAGAGAGCTTCTATGTTTTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613165435_8lhj AACAAAAACAAAAACATAGAAGCTCTCTCCAG(+AGGACA)GTACCTTACCACATTGATTGTAGACTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613165434_gnho TGGTGCTTGTTTCTCTCCGCCCACTCCAACAG(+AGGACA)GATCCTTAACACCCCCTTAATGGGGGTGGGC NGG 10 8 17 8 24 0 100 5 10