Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165501_rw2h AGAGAGCCTGCCACCACAGCTCTGGCCGTGGG(+AGGACA)GATCCTCAGAGTCTTTAGACTCTGCCTTACC NGG 10 8 17 8 24 0 100 5 10
    240613165459_va4y GAGGGGGTAAGGCAGAGTCTAAAGACTCTGAG(+AGGACA)GATCCCCACGGCCAGAGCTGTGGTGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613165458_meuq TAGTTAAAAAGAAAAAAACCTTGATACAACAG(+AGGACA)GTACCAAGAATGACTCAAACTTAAATAACAA NGG 10 8 17 8 24 0 100 5 10
    240613165457_vtre TGATATTGTTATTTAAGTTTGAGTCATTCTTG(+AGGACA)GTACCTGTTGTATCAAGGTTTTTTTCTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613165455_6ybm GCTTAAAGTCACCTACAGTTCCAGCTCCAGAG(+AGGACA)GATCCAATGCCCTCTTCTAGCCTCCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613165453_y3k7 CAGGAACCCCTGGAGGCTAGAAGAGGGCATTG(+AGGACA)GATCCTCTGGAGCTGGAACTGTAGGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240613165452_a0va CCAGTGGGTCAGAAAACCCATGCTGTATCAAG(+AGGACA)GTACCAAGATTATCGGATACTGTGAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613165451_n7iz TATAAGTCACTTCACAGTATCCGATAATCTTG(+AGGACA)GTACCTTGATACAGCATGGGTTTTCTGACCC NGG 10 8 17 8 24 0 100 5 10
    240613165449_ojh6 GCTGGGCAGGTCAGTGGCAGAGGTGGAACACG(+AGGACA)GATCCCTTCCTGACCGCGTGGTAGGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240613165448_e6qv GTCGAAAGTTACCTACCACGCGGTCAGGAAGG(+AGGACA)GATCCGTGTTCCACCTCTGCCACTGACCTGC NGG 10 8 17 8 24 0 100 5 10