Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165418_gtaf CCTGGGACATAAATGTCCCCACAGCCAGGTTG(+AGGACA)GTACCATTCAGTGTTTATTTAGACACACATA NGG 10 8 17 8 24 0 100 5 10
    240613165417_bftu ACAACCTACCTGCTCTGCTGTTTCTGGAGTTG(+AGGACA)GTACCATACAGTTCCCTGGCATGGAAGGATC NGG 10 8 17 8 24 0 100 5 10
    240613165416_xfbq GGCCCGATCCTTCCATGCCAGGGAACTGTATG(+AGGACA)GTACCAACTCCAGAAACAGCAGAGCAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613165414_44ar CATTTAGGGTACACTGGCAAAGGAAGGGGCTG(+AGGACA)GATCCTAGTTTGGCTAAAAGGCAGGCTGCAA NGG 10 8 17 8 24 0 100 5 10
    240613165413_lvec ACTCATTGCAGCCTGCCTTTTAGCCAAACTAG(+AGGACA)GATCCAGCCCCTTCCTTTGCCAGTGTACCCT NGG 10 8 17 8 24 0 100 5 10
    240613165411_9f4v AGTGTCAGGTCTGACTGCAGACGCTCTTTAGG(+AGGACA)GTACCCACTCTTCACCCTCACATAACACTAC NGG 10 8 17 8 24 0 100 5 10
    240613165409_dhv3 TGTGTGTAGTGTTATGTGAGGGTGAAGAGTGG(+AGGACA)GTACCCTAAAGAGCGTCTGCAGTCAGACCTG NGG 10 8 17 8 24 0 100 5 10
    240613165408_mok0 GAACCTGGAATAGCTGTCGGGGCTTCAGCGAG(+AGGACA)GATCCCTCCAAACCTCATGTTCTGTCCCGAT NGG 10 8 17 8 24 0 100 5 10
    240613165406_lgix GCGACATCGGGACAGAACATGAGGTTTGGAGG(+AGGACA)GATCCTCGCTGAAGCCCCGACAGCTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240613165404_k7w9 GCTCTGCAGAATCCAGCTTAATCCAGAAACAG(+AGGACA)GTACCTAAAAGAGATGGCGTCGCTCTTCTTT NGG 10 8 17 8 24 0 100 5 10