Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613165433_3ycd ACAAAGCCCACCCCCATTAAGGGGGTGTTAAG(+AGGACA)GATCCTGTTGGAGTGGGCGGAGAGAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240613165431_9m64 CAGTTTGCTTTAAATAGGTTCCACCTGCCATG(+AGGACA)GATCCATAGAGCATCTCCCAGTGGGCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613165430_z9nb CTCAGTCTCAGCCCACTGGGAGATGCTCTATG(+AGGACA)GATCCATGGCAGGTGGAACCTATTTAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613165428_ocuq GAAAGACAGGGTTGTGGTTGCTGAGCACTATG(+AGGACA)GTACCTGCCCTTAATCCCAGCACTTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613165427_axk3 CTCTTCCTCCCAAGTGCTGGGATTAAGGGCAG(+AGGACA)GTACCATAGTGCTCAGCAACCACAACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613165425_vs8z GGCAAGCTTCTACCAAGAGCAGCTTGGCCAAG(+AGGACA)GTACCTATTTGGACAAATCAGATTGACAGCA NGG 10 8 17 8 24 0 100 5 10
    240613165424_jvkt TGGTGTGCTGTCAATCTGATTTGTCCAAATAG(+AGGACA)GTACCTTGGCCAAGCTGCTCTTGGTAGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613165422_sckr TGTCTTTTTTTCACAACACTTCACCATGGTAG(+AGGACA)GTACCTACCACACCAGACTTGTCAGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613165421_1mr8 AATGCTTTTTTCTGACAAGTCTGGTGTGGTAG(+AGGACA)GTACCTACCATGGTGAAGTGTTGTGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613165419_kcfj TCTTTTATGTGTGTCTAAATAAACACTGAATG(+AGGACA)GTACCAACCTGGCTGTGGGGACATTTATGTC NGG 10 8 17 8 24 0 100 5 10